Simultaneous detection of Apple mosaic virus in cultivated hazelnuts by one-tube RT-PCR
نویسندگان
چکیده
منابع مشابه
Simultaneous detection of wheat dwarf virus, northern cereal mosaic virus, barley yellow striate mosaic virus and rice black-streaked dwarf virus in wheat by multiplex RT-PCR.
Wheat dwarf virus (WDV), barley yellow striate mosaic virus (BYSMV), rice black-streaked dwarf virus (RBSDV) and northern cereal mosaic virus (NCMV) are four viruses infecting wheat and causing similar symptoms. In this paper, a multiplex reverse transcription polymerase chain reaction (m-RT-PCR) method has been developed for the simultaneous detection and discrimination of these viruses. The p...
متن کاملDevelopment of a diagnostic one-tube RT-PCR for the detection of Rift Valley fever virus.
Diagnosis of Rift Valley fever (RVF) is based on serology and virus isolation. The disadvantages of the former include poor sensitivity, high cost, risks associated with using infectious virus as antigen, the lengthy duration of ELISA as well as cross-reactivity with other Phleboviruses. We developed, optimised and evaluated a one-tube reverse-transcription-polymerase chain reaction (RT-PCR) fo...
متن کاملDetection of Tobacco mosaic virus and Tomato mosaic virus in pepper and tomato by multiplex RT-PCR.
AIMS To develop a highly sensitive and rapid protocol for simultaneous detection and differentiation of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) in pepper and tomato. In this study, we use the multiplex PCR technique to detect dual infection of these two viruses. METHODS AND RESULTS A multiplex RT-PCR method consisting of one-tube reaction with two primer pairs targeted to re...
متن کاملMultiplex Rt-pcr Reaction for Simultaneous Detection of Tomato Torrado Virus and Pepino Mosaic Virus Co-infecting Solanum Lycopersicum
The tomato (Solanum lycopersicum L.) is cultivated all over the world and is a vegetable of significant economic importance. However, an increased production of the vegetable is directly connected with an elevated occurrence of pathogens limiting the production efficiency of the vegetable. Both, Tomato torrado virus and Pepino mosaic virus have been found to be serious disease factors. When not...
متن کاملSimultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR
A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a CTAB method. The sensitivity of the method was established using in vitro synthesized viral trans...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2010
ISSN: 1684-5315
DOI: 10.5897/ajb10.1749